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lenticrisprv2 puro vector  (Addgene inc)


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    Structured Review

    Addgene inc lenticrisprv2 puro vector
    Lenticrisprv2 Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lenticrisprv2+puro+vectors/lentiCRISPRv2+puro+(Plasmid+%2398290)/pmc13007490-34-8-10
    Average 98 stars, based on 527 article reviews
    lenticrisprv2 puro vector - by Bioz Stars, 2026-11
    98/100 stars

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    Sequencing:

    Article Title: Depleting HIF‑1α attenuates the progression of osteosarcoma, but tumorigenicity is sustained through HIF‑independent pathways.
    Article Snippet: .. After confirmation of the inserted sequence, the lentiCRISPRv2‐puro vectors were co‐transfected along with the psPAX2 (cat. no. 12260; Addgene, Inc.) and pCMV‐VSV‐G (Addgene_8454; Addgene, Inc.) vectors into Lenti‐X 293T cells (CVCL_4401; Takara Bio) using FuGENE Hd (Promega Corporation) to generate infectious lentivirus. ..

    Article Title: Depleting HIF-1α attenuates the progression of osteosarcoma, but tumorigenicity is sustained through HIF-independent pathways
    Article Snippet: .. After confirmation of the inserted sequence, the lentiCRISPRv2-puro vectors were co-transfected along with the psPAX2 (cat. no. 12260; Addgene, Inc.) and pCMV–VSV-G (Addgene_8454; Addgene, Inc.) vectors into Lenti-X 293T cells (CVCL_4401; Takara Bio) using FuGENE HD (Promega Corporation) to generate infectious lentivirus. ..

    CRISPR:

    Article Title: Lipogenic enzyme FASN promotes mutant p53 accumulation and gain-of-function through palmitoylation.
    Article Snippet: .. In brief, the sgRNAs for p53 and FASNwere designed using an online CRISPR tool69, and sgRNA sequences were inserted into the lentiCRISPRv2-blast or lentiCRISPRv2-puro vectors (Addgene). ..

    Article Title: Lipogenic enzyme FASN promotes mutant p53 accumulation and gain-of-function through palmitoylation
    Article Snippet: .. In brief, the sgRNAs for p53 and FASN were designed using an online CRISPR tool , and sgRNA sequences were inserted into the lentiCRISPRv2-blast or lentiCRISPRv2-puro vectors (Addgene). ..

    Construct:

    Article Title: RRP8, associated with immune infiltration, is a prospective therapeutic target in hepatocellular carcinoma
    Article Snippet: .. Tet-pLKO-puro (Addgene #21,915) and lentiCRISPRv2-puro vectors (Addgene #98,290) were used to construct the RRP8 shRNA and RRP8 sgRNA lentiviral plasmids, respectively. .. Plasmid DNA was extracted with a ZR Plasmid Miniprep TM -Classic kit (Zymo, D4016).

    shRNA:

    Article Title: RRP8, associated with immune infiltration, is a prospective therapeutic target in hepatocellular carcinoma
    Article Snippet: .. Tet-pLKO-puro (Addgene #21,915) and lentiCRISPRv2-puro vectors (Addgene #98,290) were used to construct the RRP8 shRNA and RRP8 sgRNA lentiviral plasmids, respectively. .. Plasmid DNA was extracted with a ZR Plasmid Miniprep TM -Classic kit (Zymo, D4016).



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